علوم زیستی دریا
Marjan Naseri Karimvand; yadollah nikpour; Ahmad Taghavi Moghadam; Kamal Ghanemi
Abstract
Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline ...
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Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline extraction.We investigated the proximate composition, gel strength, gelling point, melting point of jellyfish gelatin. Jellyfish gelatin contained 13.1% moisture, 1.3% lipid, 2.4% ash, 78.2% protein. The gelatin showed a gel strength of 33 kPa, a gelling point of 18°C, and melting point of 23°C. The gelatin was composed of α1-chain, α2-chain, β-chain, and γ-chain Jellyfish gelatin did not show superior rheological properties to mammalian gelatin, like other fish gelatin; however, it can be used in various food and cosmetic products not requiring high gel strength.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella ...
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Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella Orsini Jellyfish Venom According to Bloom method and was obtained its Concentration by Biuret method and Calculated LD50 by Jung and Choi method. According to Venom concentration and its LD50 was determined that Cause of death mice 0.5 ml of venom. The use of Na-EDTA for neutralizing venom. This Chelate Was injected In two ways to mice that In both methods, Prevented death. Na-EDTA is dedicated Chelate for Calcium excretion from body that According to nuclear calciums venom is able to separated that from Venom structure and neutralize venom.
علوم زیستی دریا
M. Parviz; yadollah nikpour; A. Taghavi Moghadam; K. Ghanemi
Abstract
In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, ...
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In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, as the other species.After extraction of nematocyst venom, the crude venom has been partially purification by sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by uv detection at 280 nm. For determine the hemolytic fraction, every fraction were injected to 3 mice via the tail vein. Finally, all data from both chromatography methods were compared. The gel filtrations first pick and the first and second pick of anion exchange chromatography showed hemolytic activity.Determination the appropriate method for purification of this venom can help to find a comprehensive method for another marine venom especially jellyfish venoms and eventually may be help to find specific antidote for jellyfish stings of these species.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate ...
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The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate the effect of temperature on the venom, it is heated at different temperatures and then injected into SoRI mice. After catching jellyfish Crambionella orsini from Arvand nidstream estuary umbrellas edge and tentacles jellyfish were separated and put were in water that was removed on the same area. Toxins LD50 were calculated by Jung and Choi method .and using statistical analysis to obtain minimal lethal dose of poison done by the 2007Excel. The results showed that the venome of jellyfish Crambionella orsini, like venome of other animals is, based on a protein and that is sensitive to heat. This venome at a temperature of 48 °C is disabled and lose their structure and also its minimum lethal dose is 0.5 ml.